Composite
pBB_RonL

Part:BBa_K1232003:Design

Designed by: Yina Xu, Austin Moon   Group: iGEM13_Washington   (2013-09-17)


Red light inducible sfGFP = PLambda-RBS-LacZ-T1-POmpC-CI-T1


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 3129
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3129
    Illegal NotI site found at 3183
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3129
    Illegal XhoI site found at 3190
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 3129
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 3129
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

None for now


Source

Jeff Tabor Plasmid - pJT106b

References

Tabor JJ, Levskaya A, Voigt CA. Multichromatic control of gene expression in Escherichia coli. J. Mol. Biol. 2011;405:315-324.